SOPs for CMD animal models
Standard protocols
Cure CMD and TREAT-NMD are collaborating in establishing standard protocols for efficacy studies in the laminin deficient mouse model LAMA2dy-W, the best described mouse model for a CMD disease, and with the aid of AFM funding, also for additional CMD animal models. With the involvement of several dedicated CMD researchers, readout parameters were selected that are appropriate to show the efficacy of a new potential drug, and that are related to patient outcomes. For each of these parameters, small groups of experts will develop a standard assessment protocol. The availability of standard protocols will implement research and increase the comparability of results.
The SOPs that have already been completed can be downloaded from this site and others will be added in the future. The last date each SOP was reviewed can be found under the code on the left of the page.
CMD mouse (CMD)
Neuromuscular junction imaging on whole muscles and muscle sections in CMD animal models
SOP Code – MDC1A_M.1.2.006
Author – Gregorius Valdez
Last update – 26 July 2016
Assessment of peripheral nerve pathology in laminin-alpha-2-deficient mice
SOP Code – MDC1A_M.1.2.001
Author – Jeffrey B. Miller
Last update – 25 June 2016
Histopathology in Hematoxylin & Eosin stained muscle sections
SOP Code – MDC1A_M.1.2.004
Author – Markus A. Ruegg
Last update – 28 January 2016
Non-invasive measurement of ankle flexion/extension torque for longitudinal follow-up of strength in small rodents
SOP Code – CMD_M.2.2.001
Author – Jean-Yves Hogrel
Last update – 16 June 2015
Single muscle contractile measurements in vivo and in situ
SOP Code – MDC1A_M.2.2.002
Author – Rich Lovering
Last update – 23 May 2015
Gait assessment in a canine model of XLMTM using an instrumented carpet
SOP Code – XLMTM_D.2.2.001
Author – Melissa Goddard
Last update – 26 March 2014
Respiratory assessment of a canine model of XLMTM using non-invasive respiratory impedence plethysmography
SOP Code – XLMTM_D.2.2.002
Author – Melissa Goddard
Last update – 26 March 2014
Histopathology in Masson Trichrome stained muscle sections
SOP Code – MDC1A_M.1.2.003
Author – Markus A. Ruegg
Last update – 28 January 2014
Inflammation quantification in the LAMA2dy-W mouse
SOP Code – MDC1A_M.1.2.005
Author – Dean Burkin
Last update – 25 January 2014
Recommendations on the care and handling of the MDC1A mouse model dyW
SOP Code – MDC1A_M.2.1.001
Author – Janice Dominov
Last update – 25 January 2014
Use of Grip Strength Meter to Assess the Limb Strength of dyw Mice
SOP Code – MDC1A_M.2.2.001
Author – Anne Connolly
Last update – 7 December 2013
Disclaimer: TREAT-NMD and the authors of the SOPs disclaim, without limitation, all liability for any loss or damage of any kind, including any direct, indirect or consequential damages, which might be incurred in connection with the information and procedures contained in these SOPs.
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